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COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE Authors: Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU, Dr. Gabriela MARCUS, student Adriana COJOCARU University of Medicine and Pharmacy Targu-Mures

COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

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Page 1: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS

ETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE

Authors:Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU, Dr. Gabriela MARCUS, student Adriana COJOCARUUniversity of Medicine and Pharmacy Targu-MuresROMANIA

Page 2: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Aim

• To assess the effect of homeopatic remedies in laboratory animals in placebo controled studies

• Do homeopatic remedies act through placebo effect?

Page 3: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

• Mice and rats from the bio-base (experimental station) of the University of Medicine and Pharmacy Targu-Mures

• Various induced experimental pathology followed by treatment

– homeopathic,

– allopathic

– combined, homeopathic and allopathic

Page 4: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ExperimentsThe effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

The antitrauma effect of the remedy Arnica in laboratory mice

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

Homeopathic treatment of grade I burns in rats with Apis and Belladonna

Page 5: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The effect of the homeopathic remedies Arnica and Staphysagria

upon the healing time of the surgical incision

Page 6: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

• Previous studies made around the world with homeopathic Arnica –contradictory results

• experimental studies with Staphysagria - missing

• What are the benefits in surgery?

• Our hypothesis – the need of administration of the remedy early after surgery

Page 7: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

• 75 Wistar rats, weight 250-270 g

• 5 groups of 15 animals

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 8: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method Surgery technique

Step 1 - tranquilization of the rats with ether

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 9: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method Surgery technique

Step 2 - preparing the surgery zone by depilation and disinfection

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 10: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method Surgery technique

Step 3 - performing a deep incision on the external side of the thigh, following the line of the femur until the appearance of the bone

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 11: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method Surgery technique

Step 4 - skin suture in 3 separate points with nonresorbable silk thread

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 12: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method TreatmentEach rat was treated through a plastic probe, starting immediately after surgery and daily administration afterwards until the complete healing of the incision

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

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Material and method Treatment

The homeopathic remedies were dissolved in water, 1 pellet in 1 ml water

• Group 1 - control group, sugar water as placebo,

• Group 2 - Arnica CH 7,

• Group 3 - Arnica CH 30,

• Group 4 - Staphysagria CH 7,

• Group 5 - Staphysagria CH 30.

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 14: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method• the length of the remaining incision was

measured each day

• the number of days necessary for the complete healing was noted for each rat

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

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ResultsThe rats treated homeopathically, both remedies and both dilutions, showed a shorter healing time (15-16 days) than the placebo group (21-22 days), with a statistically significant difference (p<0,001, t-test)

0

5

10

15

20

25

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15

Control

Arnica CH7

Arnica CH30

Staphysagria CH7

Staphysagria CH30Rat

Days for healing

The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

Page 16: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The antitrauma effect of the remedy Arnica in laboratory mice

Page 17: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The antitrauma effect of the remedy Arnica in laboratory mice

• 40 AKR2 white mice,

• 2 groups of 20 mice: control, Arnica

• Kocher clamp trauma at the level of the posterior limb– fractures

– swelling

– pain = protection of the affected limb, adynamia

• Assesment of the variation of the dorsoplantar diameter

Page 18: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Results• Control group

– Persistent swelling more than 180 minutes– adynamia– Protection of the limb

• Arnica CH7 group– Moderate swelling, gone in less than 105 minutes (p < 0,001)– Good general state– Ability to use the affected limb, even fractured– normal activity

The antitrauma effect of the remedy Arnica in laboratory mice

00,20,40,60,8

11,21,41,61,8

2

0' 15' 30' 45' 60' 75' 90' 105' 120' 135' 150' 165' 180'

Control

Arnica CH 7

mm swelling (average/group)

timpmechanic trauma

Page 19: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The compensation of muscarinic blockade provoked by atropine in

laboratory animals with the homeopathic remedy Belladonna

Page 20: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

• Pilocarpine – parasympathomimetic effect – salivation in mice

• Atropine (Atropa Belladonna) - parasympatholytic effect, blocking of the muscarinic receptors, imposibility for pilocarpine to produce salivation

• Belladonna in homeopatic dilution – does it compensate the atropinic intoxication?

Page 21: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method80 de white mice AKR2 line divided in 4 groups:

1. Assessment of the salivation produced by pilocarpine (administration of pilocarpine).

2. Assessment of the muscarinic receptors blockade by atropine, given pilocarpine (administration of atropine, then pilocarpine).

3. Assessment of supposed compensation of muscarinic blockade of atropine by homeopathic Belladonna CH7 (administration of atropine, then Belladonna CH7, then pilocarpine).

4. Control group, assessment of physiologic salivation (administration of placebo).

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 22: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and methodStep 1 – subcutaneous injection of Atropine 0,1%

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 23: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and methodStep 2 - Belladonna CH7 given by gastric probe

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 24: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and methodStep 3 – tranquilization with ethylic urethan intraperitoneally 1,5 g/kgc

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 25: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and methodStep 4 - subcutaneous injection with 1% clorhidric pilocarpine

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 26: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and methodThe mice were put on filter paper

The area of salivary impregnation was measured each 10 minutes for 30 minutes

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 27: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsThe physiologic salivation was low, nearly immeasurable on the filter paper – group 4

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 28: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsPilocarpine produces an abundant salivation (known pharmacologic effect), assessed through the saliva spot area on the filter paper – group 1

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 29: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsAtropine given before pilocarpine prevented the salivation, which was very low, nearly immeasurable on the filter paper (known pharmacologic effect) – group 2

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 30: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsIf homeopathic Belladonna CH 7 is given after atropine and before pilocarpine, the abundant salivation is present – group 3

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

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ResultsThe variation of salivation in the first 30 minutesin the 4 groups

0

20

40

60

80

100

120

140

0' 10' 20' 30'

lot 1 pilocarpinelot 3 atropine, belladonna CH7, pilocarpinelot 2 atropine, pilocarpinelot 4 physiologic salivation

mm2 saliva spot

time

(p<0,001)

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 32: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Discussions• In mice the homeopathic remedy Belladonna in CH 7 dilution

was able to give the atropinized organism the ability to be stimulated by pilocarpine.

• It is possible, in theory, that the action of the homeopathic remedy Belladonna CH 7 could be one of the following:

– it acted on the muscarinic receptors, determining them to release the atropine molecules and become sensible again to pilocarpine stimulation;

– it acted on the atropine molecules, determining them to release the receptors on which they were bound;

– it acted on cellular level, determining possible alternative ways for the de processing of the stimulus represented by pilocarpine (activation of new receptors, alternative biochemical or neuroendocrine ways)

The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

Page 33: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The effect of the homeopathic remedy Nux Vomica compared with Diazepam

on the manifestations of strychnine poisoning in laboratory mice

Page 34: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

• Stricnina (Strychnos nux-vomica L) - adynamia, tonico-clonic convulsions, hypersensitivity to sensorial stimuli, deformation of visual and acoustic perceptions, tachycardia, tachypnea, agitation and finally death.

• Diazepamul, benzodiazepină - tranquilizing, muscle-relaxant, anticonvulsant

• Nux Vomica, Strychnos nux vomica seeds dilutions –used in cases of convulsions, aggravated by external stimuli, agitation, hypersensitivity to stimuli

Page 35: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

3 groups of 20 white mice - experimental poisoning - intraperitoneal injection of 1,5 mg/kg strychnine

– Group 1 - diazepam 5 mg/kg subcutaneous 1 hour before strychnine

– Group 2 - Nux Vomica CH7 orally by plastic probe immediatelly after strychnine

– Group 3 - control (placebo), physiologic serum subcutaneous, sugar water by plastic probe

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Page 36: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method• Strychnine poisoning:

– adynamia

– tachycardia

– tachypnea

– agitation

– tonico-clonic convulsions

– death

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Page 37: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

We assessedWe assessed::

• The time until convulsionsThe time until convulsions

• The number of animals which had The number of animals which had convulsiconvulsionsons

• The time until deathThe time until death

• The number of animals which diedThe number of animals which died

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

Page 38: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsThe time until convulsionsThe time until convulsions

• 180-390 sec 180-390 sec Placebo groupPlacebo group

• 240-570 sec Diazepam240-570 sec Diazepam group group

• 660-1080 sec 660-1080 sec homeopathic Nux Vomica grouphomeopathic Nux Vomica group

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

0 200 400 600 800 1000

Placebo

Diazepam

Nux Vomica CH7

seconds until convulsions

Page 39: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Results• Convulsions present 100% in the Convulsions present 100% in the PlaceboPlacebo and and

DiazepamDiazepam group group• 70% 70% of mice had of mice had convulsconvulsions in the ions in the

homeopathic homeopathic Nux Vomica Nux Vomica groupgroup

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

0 5 10 15 20

Nux Vomica CH7

Diazepam

Placebo

convulsions no convulsions

number of mice

Page 40: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Results• Placebo groupPlacebo group micemice died in died in 240-610 sec240-610 sec• DiazepamDiazepam treated mice lived longer, treated mice lived longer, 370-685 sec370-685 sec• Homeopathic Nux Vomica treatedHomeopathic Nux Vomica treated mice showed the mice showed the

longest survivallongest survival, , 605-1150 sec605-1150 sec

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

0 200 400 600 800 1000

Placebo

Diazepam

Nux Vomica CH7

survival, seconds

Page 41: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Results• All the mice (100%) in the All the mice (100%) in the PlaceboPlacebo and and

DiazepamDiazepam group diedgroup died• HomeopaticHomeopatically ally treated mice withtreated mice with Nux Nux

VomicaVomica died in died in 80%80%

The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

0 5 10 15 20

Nux Vomica CH7

Diazepam

Placebo

died survived

Page 42: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis

and allopathic anti-inflammatory medicines in experimental acute

inflammation produced by formaldehyde

Page 43: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

• Formaldehyde – mastocyt reaction - histamine

• Apis - swelling, redness, pain, warmth

• Hydrocortisone Hemisuccinate (HHS) - steroidal anti-inflammatory

• Diclofenac - nonsteroidal anti-inflammatory

Page 44: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

• 70 white mice – local swelling in the posterior limb by subcutaneous formaldehyde 0.5%

• Dorsoplantar diameter was measured before formaldehyde and then each 15 minutes

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation

produced by formaldehyde

Page 45: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

7 groups of 10 mice

1. Hydrocortisone Hemisuccinate

2. Diclofenac

3. Apis CH 7

4. Apis CH 15

5. Hydrocortisone Hemisuccinate and Apis CH 7

6. Hydrocortisone Hemisuccinate and Apis CH 15

7. Control group, placebo

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation

produced by formaldehyde

Page 46: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Results

• Local signs of inflammation– redness

– swelling

– motor impairment

– agitation

– protection of the limb

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation

produced by formaldehyde

Page 47: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

ResultsDorsoplantar diameter evolution in time (average values)

Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation

produced by formaldehyde

0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0' 15' 30' 45' 60' 75' 90' 105' 120' 135' 150' 165' 180'

Control - PlaceboDiclofenacHHSApis CH 15HHS + Apis CH 7Apis CH 7HHS + Apis CH 15

mm swelling

timp

formaldehyde, Apis

HHS, Diclofenac

Page 48: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Homeopathic treatment of grade I burns in rats with Apis and Belladonna

Page 49: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Homeopathic treatment of grade I burns in rats with Apis and Belladonna Material and method

• 3 groups of 10 rats

• Tranquilization with ether

• Immersion of posterior left limb in hot water at 55°C for 20 seconds

Page 50: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

Grade I burns - edema and erithema

Page 51: COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE

Material and method

Treatment by gastric probe:

• Group 1 - placebo as sugar water

• Group 2 - Apis CH 9

• Group 3 - Belladonna CH 9

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Material and methodAssessment:

– The number of healed rats each day

– The average number of days necessary for the complete healing for each group

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Results

• Apis CH 9 group showed the fastest healing, followed by the Belladonna CH 9 group

• The Placebo group showed the longest healing time

0 1 2 3 4

Apis CH 9

BelladonnaCH 9

Placebo

average, day for healing

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Results

• Apis group healed in the days 1-2, with 80% rats in the first day

• Belladonna group healed in the days 1-3

• Placebo group healed in the days 2-4 0

2

4

6

8

10

12

Day 1 Day 2 Day 3 Day 4

Apis CH 9

Belladonna CH 9

Placebo

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Final conclusions

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Conclusions (1/4)

• The homeopathic remedies Arnica and Staphysagria in the dilutions CH 7 and CH 30, given early after surgery, had a significant effect in the reduction of the healing time of the surgical incision in rats

• The homeopathic remedy Arnica CH 7 fights the effects of mechanic trauma in mice if given immediately after trauma

• In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the capability to be stimulated by pilocarpine, even if the specific receptors were blocked

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Conclusions (2/4)

• Homeopatic Nux Vomica CH7 was significantly more efficient than Diazepam in delaying and even preventing the convulsions in the experimental strychnine poisoning. The time until death was significantly prolonged, some of the mice treated homeopathically surviving the experiment

• The anti-inflammatory effect of the remedy Apis in our experiments had a higher intensity than Hydrocortisone Hemisuccinate and Diclofenac, without being antidoted by them in combinations

• Apis CH 9 wad more efficient than Belladonna CH 9 in the treatment of grade I burns in rats, both being more efficient than placebo

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Conclusions (3/4)

In all our experiments, the effect of homeopathic remedies was objectively assessed, reproducible and statistically significant

Homeopathy is different from placeboHomeopathy is different from placebo

Homeopathy acts quickly in acuteHomeopathy acts quickly in acute

Homeopathy can be associated with allopathyHomeopathy can be associated with allopathy

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Conclusions (4/4)

Homeopathic research must be extendedHomeopathic research must be extended

C'est la guerre!

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Thank you for you attention