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Carrez Clarification Reagent Kit

Catalog Number MAK191Store at Room Temperature

TECHNICAL BULLETIN

Product DescriptionThe Carrez Clarification Reagent Kit is suitable for thepreparation of samples for the analysis of smallmolecules such as carbohydrates, alcohols, aldehydes,and organic acids. The samples might contain proteins,fats, and redox compounds that interfere with theanalysis. Carrez Clarification Reagents remove theseinterfering compounds from the analytes and are moresimple, less expensive, and less time-consuming thanusing spin filters.

The Carrez Clarification Reagent Kit can be used forthe preparation of food (such as meat, cereal, or milk)or blood for further analysis (such as HPLC orbioassays). The reagents in this kit cannot be used toprepare samples that contain or may be converted toascorbate, ammonia, or citrate in enzyme-basedassays.

ComponentsThe kit reagents are sufficient for clarification of100 samples.

Carrez Reagent I 500 µLCatalog Number MAK191A

Carrez Reagent II 500 µLCatalog Number MAK191B

Reagents and Equipment Required but NotProvided

• 0.22 µm syringe filters (optional for samples with

low analyte concentrations)• Buffer, pH ∼8.0 (optional for samples with low

analyte concentrations)

Precautions and DisclaimerThis product is for R&D use only, not for drug,household, or other uses. Please consult the SafetyData Sheet for information regarding hazards and safehandling practices.

Preparation InstructionsBriefly centrifuge vials before opening. Use ultrapurewater for the preparation of reagents.

Carrez Reagents I and II are ready to use solutions.

Storage/StabilityThe kit is shipped at ambient temperature and storageat room temperature is recommended.

Exposure of Carrez Reagent I to strong acids must beavoided as it may produce toxic cyanide gas.

Procedure

Homogenize 10 mg of the sample in 100 µL of water toobtain a uniform suspension. Transfer the suspensionto a microcentrifuge tube.

 Add 5 µL of Carrez Reagent I to the suspension and

mix thoroughly by vortexing.

 Add 5 µL of Carrez Reagent II to the mixture and vortexuntil homogenous.

Centrifuge at 10,000 ×  g  for 2 minutes. Transfer theclear supernatant to fresh tubes.

Dilute samples with high analyte concentrations100-fold using water before use.

Samples with low analyte concentrations may beprocessed with minimal dilution by precipitating the

excess zinc ions from the Carrez Reagent II. Adjust thepH of the sample to ∼8.0 by adding buffer atapproximately 1:1 ratio with the sample. Centrifuge

briefly or filter through a 0.22 µm syringe filter toremove Zn(OH)2 precipitate.

GD,KVG,LS,MAM 09/14–1

©2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of Sigma-Aldrich Co. LLC, registered in the US and other

countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for theirparticular use. Additional terms and conditions may apply. Please see product information on the Sigma-Aldrich website at

www.sigmaaldrich.com and/or on the reverse side of the invoice or packing slip.